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HRP Conjugated Arachis

HRP Conjugated Arachis

Posted on November 27, 2021 by Alan

Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilized, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Biochem/Physiol Actions

PNA would not agglutinate common human erythrocytes, nevertheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very similar to the anti-T antibody in human sera. The lectin will be utilized to inform aside between human lymphocyte subsets.

Abstract

Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker  it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.

For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity.

 

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is obtainable in a stabilized liquid kind.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) tools (SKU: 20810000). ICL is a two-component substrate that accommodates a gentle luminol reply with an enhancer and a gentle peroxide buffer reply.

arachis eylabs
arachis eylabs

It has a robust light emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over totally different detection methods incorporates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an acceptable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one ingredient peroxidase substrate (SKU: 21530073).

Software program

Frequent Western Blot Protocol:

  • Glycoprotein sample dimension: 500ng
  • Lectin Focus: 0.1ug/ml
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS net web page gel
  3. Change gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Unit Definition

One unit will kind 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Bodily kind

Accommodates sodium citrate

Preparation Discover

Prepared from peroxidase (P8375) using a modification of a broadcast method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Arachis hypogaea lectin (PNA)

05-0116-10 Medicago 10 mg
EUR 139.2

Arachis hypogaea lectin (PNA

05-0116-1000 Medicago 1 g Ask for price

Arachis hypogaea lectin (PNA

05-0116-50 Medicago 50 mg
EUR 376.8

Arachis hypogaea Arachin Ahy-3

1-CSB-EP737391ANE Cusabio
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli

Arachis hypogaea Arachin Ahy-3

1-CSB-YP737391ANE Cusabio
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-YP331779ANE(A4) Cusabio
  • EUR 703.20
  • EUR 358.80
  • EUR 2606.40
  • EUR 1080.00
  • EUR 1730.40
  • EUR 458.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P41B

1-CSB-CF337356ANE Cusabio
  • EUR 1158.00
  • EUR 798.00
  • EUR 858.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-EP331779ANE(A4) Cusabio
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-EP331779ANE Cusabio
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli

Asprosin Polyclonal Antibody (Human), HRP

4-PAA332Hu01-HRP Cloud-Clone
  • EUR 475.20
  • EUR 4801.20
  • EUR 1312.80
  • EUR 614.40
  • EUR 289.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Asprosin. This antibody is labeled with HRP.

Vinculin (VCL) Polyclonal Antibody (Human), HRP

4-CAB839Hu01-HRP Cloud-Clone
  • EUR 345.60
  • EUR 2958.00
  • EUR 852.00
  • EUR 430.80
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with HRP.

Catalase (CAT) Monoclonal Antibody (Human), HRP

4-MAC418Hu22-HRP Cloud-Clone
  • EUR 345.60
  • EUR 2958.00
  • EUR 852.00
  • EUR 430.80
  • EUR 234.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Catalase (CAT). This antibody is labeled with HRP.

Catalase (CAT) Monoclonal Antibody (Human), HRP

4-MAC418Hu23-HRP Cloud-Clone
  • EUR 298.80
  • EUR 2293.20
  • EUR 685.20
  • EUR 363.60
  • EUR 213.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Catalase (CAT). This antibody is labeled with HRP.

Ferroportin (FPN) Monoclonal Antibody (Human), HRP

4-MAC489Hu24-HRP Cloud-Clone
  • EUR 386.40
  • EUR 3537.60
  • EUR 996.00
  • EUR 488.40
  • EUR 252.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Ferroportin (FPN). This antibody is labeled with HRP.

Reelin (RELN) Monoclonal Antibody (Human), HRP

4-MAC775Hu21-HRP Cloud-Clone
  • EUR 396.00
  • EUR 3668.40
  • EUR 1029.60
  • EUR 501.60
  • EUR 254.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Human Reelin (RELN). This antibody is labeled with HRP.

Reelin (RL) Monoclonal Antibody (Rat), HRP

4-MAC775Ra21-HRP Cloud-Clone
  • EUR 411.60
  • EUR 3894.00
  • EUR 1086.00
  • EUR 524.40
  • EUR 261.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against Rat Reelin (RL). This antibody is labeled with HRP.
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