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Toxo IgG ELISA Kit

Posted on May 11, 2021May 11, 2021 by Alan

An enzyme-linked immunosorbent assay for the qualitative determination of IgG anti-Toxoplasma Gondii (T. Gondi) in human serum or plasma.

Each kit contains:

  • Recombinant Toxo Antigens Coated Microwells (8 wells x 12 strips)
  • 0.5 mL Toxo IgG Negative Control
  • 0.5 mL Toxo IgG Positive Control
  • Sample diluent 11 mL
  • HRP-conjugated anti-human IgG 11 mL
  • Wash buffer (30 x concentrate) 20 mL
  • Substrate TMB A 6 mL
  • Substrate TMB B 6 mL
  • Stop solution 6 mL
  • ELISA worksheet 2 sets
  • Product insert 1 set

T. Gondi is an obligate intracellular protozoan parasite with worldwide distribution (1,2). Serological data indicate that approximately 30% of the majority population industrialized nations are chronically infected with the organism (3). When a If a seronegative woman becomes infected with T. Gondi during pregnancy, the body becomes it is often transmitted through the placenta to the fetus (1,4).

The severity of the infection in the fetus varies with the trimester during which the infection was acquired, Infection during the trimester can lead to miscarriage, fetal death, or overt disease in the neonate. Approximately 75% of newborns with congenital infection are symptomatic. However, almost all children born with subclinical toxoplasmosis will develop adverse effects. Ocular or neurological sequelae in later life (4,7).

About 80-85% develop chorioretinitis and some may also experience blindness or mental retardation. Toxoplasmosis can be treated with antibiotics, but early detection of the disease is crucial for effective therapy.

A variety of serologic tests for antibodies to T. Gondi have been used to aid in the diagnosis of acute infection and to assess previous exposure to the organism. The most commonly used tests include the Sabin-Feldman stain test, direct agglutination,
Indirect hemagglutination, latex agglutination, indirect immunofluorescence and ELISA (5,6).

The Toxo IgG ELISA Kit is a new generation of ELISA tests. The test uses multiple Recombinant proteins to minimize false-positive reactions caused by cross-reaction to the native Toxo antigen used in the native antigen-based ELISA.

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