Bile acids (BA) make up 67% of the total composition of bile. They are 24-carbon steroids generated during cholesterol metabolism. They form conjugates with glycine or taurine to form bile salts. Five of the BAs represent more than 99% of the total population found in biofluids. The average composition in healthy individuals includes conjugates of cholic, deoxycholic, and lithocholic acids. Bile acids are critical due to their ability to solubilize lipids by forming micelles with cholesterol and fatty acids.
Their synthesis is not only essential for the elimination of cholesterol from the body, but they are also necessary for the proper absorption of dietary lipids in the small intestine. Measurement of circulatory Total Bile Acids (TBA) therefore provides information on liver functions and liver diseases such as jaundice and hepatocellular injury. TBA estimation can detect liver damage during the early stages and allows patients to receive treatment before liver damage becomes irreversible.
In addition, bile acids participate as signaling molecules that interact with G-protein-coupled receptors (GPCR), TGR5, and the nuclear farnesoid receptor X (FXR). The BioVision Total Bile Acid Assay Kit provides a simple, sensitive, and high throughput adaptable approach to detect the physiological concentration of total bile acids in a variety of biological fluids. The principle of the assay is based on an enzymatic cycling method in the presence of NADH and a chromophore.
The reduction of the chromophore produces a stable colorimetric product whose absorbance can be kinetically tracked at 405 nm. This absorbance is directly proportional to the amount of TBA in the sample. Our essay is very specific and sensitive. Other metabolites found in biofluids do not interfere with the assay. The assay can detect as little as 1 µM of bile acids in a variety of samples.
Features & benefits:
- Cat # + Size K209-100
- Size 100 trials
- Detection method Absorbance (405 nm).
- Species Reactivity Mammal
- Applications Estimation of Bile Acids in various biological samples.
- Simple, fast, and convenient testing
- Can detect bile acids as low as 1 µM in a variety of samples.